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Clinical Chemistry 12: 90-94, 1966;
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Clinical Chemistry, Vol 12, 90-94, Copyright © 1966 by the American Association for Clinical Chemistry

Determination of Serum Calcium by Glyoxal Bis (2-Hydroxyanil) Chelation

John F. Bellinger 1 and Robert A. Campbell 2

1 Department of Pediatrics, University of Oregon Medical School, Portland, Ore. 97201.
2 Department of Pediatrics, University of Oregon Medical School, Portland, Ore. 97201.

A simple micro (0.1 ml.) colorimetric method is described for the determination of serum calcium. This method employs the differential chelating characteristic of glyoxyl bis (2-hydroxyanil) in the presence of other divalent cations. The serum sample is placed in a colorimetric tube followed by additions of water, glyoxyl bis (2-hydroxyanil) reagent, and alkaline carbonate color developer. Chloroform is added to extract the chromogen, and its transmittance at 535 mµ is determined.

Submitted on May 17, 1965
Accepted on November 5, 1965






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Copyright © 1966 by the American Association for Clinical Chemistry.