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Clinical Chemistry 12: 151-157, 1966;
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Clinical Chemistry, Vol 12, 151-157, Copyright © 1966 by the American Association for Clinical Chemistry

Use of the Berthelot Reaction in the Automated Analysis of Serum Urea Nitrogen

Alan A. Wilcox 1, Wallace E. Carroll 1, Rex E. Sterling 1, H. A. Davis 1, and Arnold G. Ware 1

1 Main Laboratory, Los Angeles County General Hospital, and the Department of Biochemistry, University of Southern California School of Medicine, Los Angeles, Calif. 90033

A method is presented for the automated determination of urea nitrogen using the Berthelot reaction for measuring the ammonia released by the enzymatic action of urease. The method compares favorably with the automated diacetyl monoxime method and employs less dangerous and less expensive reagents.

Submitted on August 18, 1965
Accepted on November 27, 1965







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Copyright © 1966 by the American Association for Clinical Chemistry.