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Clinical Chemistry 12: 187-193, 1966;
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Clinical Chemistry, Vol 12, 187-193, Copyright © 1966 by the American Association for Clinical Chemistry

Colorimetric Determination of Serum Guanase Activity

Wendell T. Caraway 1

1 Laboratories of McLaren General Hospital, St. Joseph Hospital, and the Flint Medical Laboratory, Flint, Mich.

A colorimetric method for the determination of serum guanase has been developed whereby ammonia, formed by the deteramination of guanine, is measured by the phenatehypochlorite reaction. Variations in substrate concentration, time of incubation, pH, temperature, and concentration of enzyme have been investigated with respect to enzyme activity. The determination of serum guanase appears to be useful in the differential diagnosis of jaundice.

Submitted on August 16, 1965
Accepted on January 3, 1966






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Copyright © 1966 by the American Association for Clinical Chemistry.