Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 12: 194-205, 1966;
This Article
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fernandez, A.
Right arrow Articles by Goldenberg, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fernandez, A.
Right arrow Articles by Goldenberg, H.

Clinical Chemistry, Vol 12, 194-205, Copyright © 1966 by the American Association for Clinical Chemistry

An Improved Method for Determination of Serum Albumin and Globulin

Alberto Fernandez 1, Charles Sobel 1, and Harry Goldenberg 1

1 Bio-Science Laboratories, 12330 Santa Monica Blvd., Los Angeles, Calif. 90025.

A simple and reliable method has been developed for the analysis of serum proteins. Globulins are precipitated from serum with HCI- ethanol. The albumin remains in the supernatant fluid. The albumin fraction is then precipitated by alcoholic sodium acetate. The proteins are redissolved and determined colorimetrically following their reaction with the biuret reagent.

Recovery and electrophoretic studies indicate that the method affords a clean-cut separation of albumin from the globulin fraction. Lipemia does not appear to interfere with the analysis.

Submitted on April 10, 1964
Accepted on December 26, 1965






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1966 by the American Association for Clinical Chemistry.