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Clinical Chemistry 12: 258-262, 1966;
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Clinical Chemistry, Vol 12, 258-262, Copyright © 1966 by the American Association for Clinical Chemistry

Quantitative Analysis of Urinary 17-Oxogenic Steroids Using Sodium Metaperiodate Oxidation

Mary G. Mefcalf 1

1 Medical Unit, The Princess Margaret Hospital, Christchurch, New Zealand.

At 550° the oxidation of pregnane-17, 20-diols (17- oxogenic steroids) to 17-oxosteroids with sodium metaperiodate is complete within 10 mm. The yield of 17-oxosteroids is not affected by light or pH's between 4.6 and 7.0. The 17-oxgenic steroids may be oxidized in the presence of glucose provided that additional sodium metaperiodate, equivalent in amount to or in excess of the glucose, is used for oxidation.

Submitted on October 11, 1965
Accepted on January 4, 1966




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Copyright © 1966 by the American Association for Clinical Chemistry.