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Clinical Chemistry 12: 350-359, 1966;
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Clinical Chemistry, Vol 12, 350-359, Copyright © 1966 by the American Association for Clinical Chemistry

Human Plasma and Serum Trypsin-like Esterase Activity

Roman B. Rutkowski 1

1 Division of Biochemistry, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C. 20012.

A spectrophotometric method using benzoyl arginine ethyl ester (BAEE) is described for the assay of serum and plasma trypsin-like esterase activity. The method was used to find activities in serum and plasma (anticoagulated with oxalate and heparin) from 12 healthy males at 3 different reaction pH's.

The effects of calcium, oxalate, heparin, soybean trypsin inhibitor, ovomucoid, and heat on this activity were also measured. Values of esterase activity were significantly different, indicating a variable enzyme or inhibitor composition in the 3 sets of samples. Plasma from heparinized blood gave unique activity responses to pH and to the agents tested for effect.

Accepted on March 9, 1966






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Copyright © 1966 by the American Association for Clinical Chemistry.