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Clinical Chemistry 12: 369-375, 1966;
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Clinical Chemistry, Vol 12, 369-375, Copyright © 1966 by the American Association for Clinical Chemistry

Microdetermination of Protein Nitrogen with a Dumbbell Diffusion Apparatus

Deana Nobel 1

1 Department of Biophysics, University of Pittsburgh, Pittsburgh, Pa. Supported by Grant GM 10403-03 from the U.S. Public Health Service.

The use of a two-chamber, sealed microdiffusion apparatus has been investigated for the determination of protein nitrogen on samples containing 0.5 mg. protein. The apparatus is constructed from a ground-glass ball-and-socket joint, and all operations including digestion are performed in the designed apparatus. The method has been applied to the determination of nitrogen in tobacco mosaic virus protein and found to be in good agreement with theoretical values.

Submitted on July 15, 1965
Accepted on January 7, 1966






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Copyright © 1966 by the American Association for Clinical Chemistry.