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Clinical Chemistry 12: 399-405, 1966;
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Clinical Chemistry, Vol 12, 399-405, Copyright © 1966 by the American Association for Clinical Chemistry

The Quantitation of Major 17-Ketosteroid Fractions by Gas-Liquid Chromatography

Charles R. Berrett 1 and Crichton McNeil 1

1 Holy Cross Hospital Research Foundation, Salt Lake City, Utah 84102.

A method for measuring the major urinary 17-ketosteroid fractions has been developed. This method includes extraction of the conjugated steroids, hydrolysis, extraction of the free steroids, purification of the extraction, and quantitation of the steroids contained in the purified extract by gas-liquid chromatography. Data are presented on the excretion of androsterone, etiocholanolone, and dehydroepiandrosterone by normal male and female subjects. The procedure is discussed with regard to accuracy, reproducibility, precision, time per analysis, and correlation with the level of total Zimmerman chromogens in the urine.

Submitted on October 4, 1965
Accepted on March 11, 1966




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Copyright © 1966 by the American Association for Clinical Chemistry.