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Clinical Chemistry 12: 482-490, 1966;
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Clinical Chemistry, Vol 12, 482-490, Copyright © 1966 by the American Association for Clinical Chemistry

Phenolphthalein Monophosphate as a Substrate for Serum Alkaline Phosphatase

Arthur L. Babson 1, Sharon J. Greeley 1, Charles M. Coleman 1, and George E. Phillips 1

1 Biochemistry Department, Warner-Lambert Research Institute, Morris Plains, N.J.

A rapid and precise procedure for serum alkaline phosphatase employs phenolphthalein monophosphate as the substrate. This compound is readily hydrolyzed and directly provides a chromogen for colorimetric determination. The color produced is linear with respect to both enzyme concentration and incubation time and is stable. Reagent and serum blanks are negligible. Various factors which affect alkaline phosphatase activity have been evaluated. The importance of the proper selection of buffer species, concentration, and pH is emphasized.

Submitted on December 23, 1965
Accepted on May 24, 1966




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