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Clinical Chemistry 12: 497-504, 1966;
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Clinical Chemistry, Vol 12, 497-504, Copyright © 1966 by the American Association for Clinical Chemistry

Enzyme Fractionation by Means of Large- Scale Continuous- Flow Electrophoresis

Seymour Winsten 1, Jeanne Jackson 1, and Paula Wolf 1

1 Department of Laboratories, Albert Einstein Medical Center, Philadelphia, Pa.

Large- scale, microglass bead, continuous- flow electrophoresis was used to separateand collect protein fractions from a single serum sample. These fractions were assayed for a multiplicity of enzyme activities. Relatively good recovery was obtained with lactic acid dehydrogenase, leucine aminopeptidase, and phosphohexoseisomerase. Artifactually high recoveries were observed with aldotase and isocitric acid dehydrogenase. The increase observed in aldolase activity may be due to a pH effect.

Submitted on October 27, 1965
Accepted on May 9, 1966







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Copyright © 1966 by the American Association for Clinical Chemistry.