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Clinical Chemistry 12: 509-512, 1966;
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Clinical Chemistry, Vol 12, 509-512, Copyright © 1966 by the American Association for Clinical Chemistry

Effect of a Nonionic Detergent on the Spectral Absorption of Oxyhemoglobin in the Presence of Bilirubin

Sanford H. Jackson 1

1 Research Institute and the Division of Clinical Chemistry of The Hospital for Sick Children, Toronto, Canada.

The presence of the nonionic detergent Brij 35 in a solution of oxyhemoglobin and bilirubin has been found to change the spectral transmission curve of the oxyhemoglobin. The major change is in the 475 mµ region, where the transmission is greatly increased. A combination of the bilirubin with the oxyhemoglobin by an intermediate linkage with the Brij is postulated.

Submitted on January 11, 1966
Accepted on March 14, 1966






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Copyright © 1966 by the American Association for Clinical Chemistry.