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Clinical Chemistry 12: 559-569, 1966;
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Clinical Chemistry, Vol 12, 559-569, Copyright © 1966 by the American Association for Clinical Chemistry

A Gas Chromatographic Method for the Determination of Lactic Acid in Blood

John Savory 1 and Alex Kaplan 2

1 University of Florida, College of Medicine, Gainesville, Fla.
2 University Hospital and Department of Biochemistry, University of Washington, Seattle, Wash. 98105.

A method for the determination of lactic acid concentration in blood by gas chroma tography is described. Lactic acid in a protein-free filtrate is oxidized by ceric sulfate to acetaldehyde. The latter compound is introduced into the gas chroma tographic column by vapor phase injection, and is quantitatively measured within 3 min. The method is specific, precise, and not affected by compounds that may interfere with the determination of lactic acid by colorimetric or enzymatic methods.

A method for preserving blood samples up to 24 hr. without change in lactic acid concentration is also described.

Accepted on February 17, 1966




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Copyright © 1966 by the American Association for Clinical Chemistry.