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Clinical Chemistry 12: 596-605, 1966;
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Clinical Chemistry, Vol 12, 596-605, Copyright © 1966 by the American Association for Clinical Chemistry

Thin-Layer Starch-Gel Electrophoresis

A Simple, Accurate Method for Characterization and Quantitation of Protein Components

Lena A. Lewis 1

1 Research Division, The Cleveland Clinic Foundation, Cleveland, Ohio 44106

A modification of the starch-gel electrophoresis technic of Smithies is presented,which enables quantitation of the resolved fractions to be carried out with little equipment other than that used for paper electrophoretic fractionation of proteins.A sheet of plastic (Stabilene film) wrapped around a kymograph type drum is evenly coated with starch-gel. The sheet is suspended in an "open strip" Durrum type cell and electrophoresis carried out. After staining with suitable stain to demonstrate proteins, lipids, or other components, the patterns are scanned in a photoelectric densitometer. Results obtained with this procedure show good reproducibility when fractionating solutions of hemoglobin and serum proteins.

Submitted on April 21, 1966
Accepted on June 16, 1966






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Copyright © 1966 by the American Association for Clinical Chemistry.