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Clinical Chemistry 12: 606-612, 1966;
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Clinical Chemistry, Vol 12, 606-612, Copyright © 1966 by the American Association for Clinical Chemistry

The Automated Determination of Acetoacetate in Serum and Urine

Bernard Klein 1 and Morris Oklander 1

1 Automation Research Laboratory, Veterans Administration Hospital, Bronx, N. Y. 10468.

A procedure based on the Rothera reaction is presented for the automated determination of acetoacetate in serum and urine. The mean serum acetoacetate concentration determined by this procedure for normal human subjects is 0.20 mM; S.D. is 0.059 mM. Recovery experiments demonstrate excellent reliability. The interferences are identified and their effects evaluated.

Submitted on March 8, 1966
Accepted on June 20, 1966






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Copyright © 1966 by the American Association for Clinical Chemistry.