Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 13: 19-25, 1967;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Preston, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Preston, J. A.

Clinical Chemistry, Vol 13, 19-25, Copyright © 1967 by the American Association for Clinical Chemistry

3-Methoxy-4-Hydroxymandelic Acid (VMA) by Microfiber Chromatography

Joseph A. Preston 1

1 Colorado Pathologists Reference Laboratory, 1634 Downing St., Denver, Cob. Requests for reprints should be directed to this address.

A method is presented for the rapid, precise, quantitative measurement of 3-methoxy-4-hydroxymandelic acid (VMA) in urine. The method is unique in that it utilizes silica-gel-impregnated glass fiber as a support medium for thin-layer chromatography. An ethyl acetate extract of acidified urine was spotted and chromatographed in an ascending technic using tolulene, glacial acetic acid, and ethyl acetate (70:20:5) as a solvent system. The VMA is identified by spraying the chromatogram with diazotized p-nitroanaline. Semiquantitation was made with accuracy by visual inspection and comparison with simultaneously spotted standards. Precise quantitation was made by strip scanning. Diet and a variety of medication did not interfere. The normal range was found to be 0-4 mg./L.

Submitted on June 14, 1966
Accepted on August 9, 1966




The following articles in journals at HighWire Press have cited this article:


Home page
 Arch SurgHome page
J. G. Batsakis, G. L. Brody, J. D. Bartlett, and D. E. Rice
Pheochromocytoma of the Bladder: Detection by a New Screening Test
Arch Surg, February 1, 1968; 96(2): 254 - 258.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1967 by the American Association for Clinical Chemistry.