Clinical Chemistry
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Clinical Chemistry 13: 2-5, 1967;
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Clinical Chemistry, Vol 13, 2-5, Copyright © 1967 by the American Association for Clinical Chemistry

Kinetic Aspects of Cyanmethemoglobin Formation from Carboxyhemoglobin

F. Lee Rodkey 1

1 Laboratory of Analytical Biochemistry, U. S. Naval Medical Research Institute, National Naval Medical Center, Bethesda, Md. 20014.

The conversion of carboxy hemoglobin (HbCO) to cyanmethemoglobin (MHbCN) by Drabkin's reagent has been shown to be considerably slower than is the same reaction with oxyhemoglobin(Hb02). Conversion of Hb02 to MHbCN is complete in 10 min. whereas total conversion of HbCO requires 90-120 min. This difference in reaction rate must be considered in the determination of total hemoglobin as MHbCN when the blood contains appreciable HbCO.

Submitted on July 14, 1966
Accepted on September 1, 1966






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Copyright © 1967 by the American Association for Clinical Chemistry.