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Clinical Chemistry 13: 49-54, 1967;
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Clinical Chemistry, Vol 13, 49-54, Copyright © 1967 by the American Association for Clinical Chemistry

A Modified Copper Method for the Estimation of alpha-Amino Nitrogen in Urine

C. C. Clayton 1 and Betty F. Steele 2

1 Department of Biochemistry, Medical College of Virginia, Richmond, Va. 23219.
2 208 Henry St., Ann Arbor, Mich.

The copper method of Pope and Stevens (1) for estimation of alpha-amino N has been modified by a change in buffer and the use of tetraethylenepentamine as a color intensifier, giving a good colorimetric assay. alpha-Amino N was measured successfully in concentrations of 0.2-2.8 mg./10 ml. Interference due to ammonia has been minimized, and interference due to certain other materials has been determined with suggestions for its elimination. The modified procedure offers the advantages of simplicity and speed in obtaining an approximate determination of alpha-amino N in urine.

Submitted on July 28, 1966
Accepted on September 20, 1966






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Copyright © 1967 by the American Association for Clinical Chemistry.