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Clinical Chemistry 13: 834-846, 1967;
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Clinical Chemistry, Vol 13, 834-846, Copyright © 1967 by the American Association for Clinical Chemistry

An Enzymatic Spectrophotometric Method for the Determination of Xanthine and Hypoxanthine

James R. Klinenberg 1, Stephen Goldfinger 2, Kathryn H. Bradley 3, and J. Edwin Seegmiller 3

1 Department of Medicine, UCLA Medical Center, Los Angeles, Calif. 90024.
2 Department of Medicine, Massachusetts General Hospital, Boston, Mass.
3 National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, U. S. Public Health Service, Bethesda, Md. 20014.

A method for determining the total content of the oxypurines xanthine and hypoxanthine in urine is described which is technically simplified and has greater specificity than existing methods. This method is highly specific, reproducible, and accurate. It also allows an estimation of the present xanthine and hypoxanthine in the urine without performing any additional measurements. When applied to plasma, special concentrating procedures are required and the method is therefore less precise.

Submitted on December 27, 1966
Accepted on March 20, 1967




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Copyright © 1967 by the American Association for Clinical Chemistry.