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Clinical Chemistry 13: 847-854, 1967;
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Clinical Chemistry, Vol 13, 847-854, Copyright © 1967 by the American Association for Clinical Chemistry

Reaction Rate Measurement of Multiple Enzyme Samples by Continuous Flow Analysis

Harold H. Brown 1 and Mary R. Ebner 1

1 Harrisburg Polyclinic Hospital, Harrisburg, Pa. 17105.

A simple technic to adapt the advantages of continuous flow analysis to the kinetic assay of multiple enzyme samples is described. It will permit adequate standardization by primary or secondary standards run through the entire analysis of those procedures that do not exhibit spontaneous changes in light absorption or fluorescence with time. The adaptation of the Kind-King method for alkaline phosphatase (1) to this technic is given to demonstrate the superiority of such a system over single-point enzyme assays.

Submitted on February 2, 1967
Accepted on March 18, 1967






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Copyright © 1967 by the American Association for Clinical Chemistry.