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Clinical Chemistry 13: 877-889, 1967;
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Clinical Chemistry, Vol 13, 877-889, Copyright © 1967 by the American Association for Clinical Chemistry

Measurement of Total Body Hemoglobin by Bloodless Technic

Bernard J. Katchman 1, James P. F. Murphy 1, Kenneth M. Offner 1, and Jerome C. Fox 1

1 Department of Research, Miami Valley Hospital, 1 Wyoming St., Dayton, Ohio 45409.

The theory and methodology for the determination of total body hemoglobin by Sjostrand's closed breathing technic has been evaluated. The results show that it is possible to determine total body hemoglobin from the measurement of alveolar carbon monoxide concentrations. A system for closed breathing measurement was designed and assembled which permits continuous monitoring of alveolar carbon monoxide and oxygen. Improvements in this system are suggested such that total body hemoglobin may be read out directly, from the determination of alveolar carbon monoxide concentrations, by means of a sensitive infrared analyzer. Such measurements of total body hemoglobin have a high correlation coefficient with measurements of total body hemoglobin made by the Risa (radioiodinated serum albumin) blood volume technic.

Submitted on September 30, 1966
Accepted on March 13, 1967






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Copyright © 1967 by the American Association for Clinical Chemistry.