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Clinical Chemistry 13: 1017-1020, 1967;
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Clinical Chemistry, Vol 13, 1017-1020, Copyright © 1967 by the American Association for Clinical Chemistry

Improving the Specificity of the Nitrosonaphthol Procedure for Urinary 5-Hydroxyindoleacetic Acid

A. H. Anton 1 and D. F. Sayre 1

1 Departments of Anesthesiology and of Pharmacology, University of Florida College of Medicine, Gainesville, Fla.

The nitrosonaphthol procedure for urinary 5-HIAA has been modified by using an excess of NaCl and adjusting the urine to a constant pH during the first chloroform purification step. Thus, the variable salt concentration of different urine samples, which has been claimed to be responsible for the reported inconsistency of the method, has been eliminated.

Submitted on January 31, 1967
Accepted on March 17, 1967






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Copyright © 1967 by the American Association for Clinical Chemistry.