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Clinical Chemistry 13: 947-952, 1967;
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Clinical Chemistry, Vol 13, 947-952, Copyright © 1967 by the American Association for Clinical Chemistry

Microestimation of Fibrinogen with a Semimicro Modification Applicable to Icteric Plasma

Jesse F. Goodwin 1

1 Clinical Research Center, Children's Hospital of Michigan, and Wayne State University School of Medicine, Detroit, Mieh. 48202.

The sulfite precipitation technic for fibrinogen has been modified to assay micro-volumes of plasma and eliminate interference from bilirubin. The precipitated fibrinogen is dissolved in urea and reacted with a modified biuret reagent. The resulting biuret complex may be read at 320 or 540 mµ. Bilirubin interference is eliminated by an oxidation step employing NaOCl and citric acid. The values obtained by the modified technics compare favorably with each other and with the macroprocedure.

Submitted on March 6, 1967
Accepted on April 11, 1967






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Copyright © 1967 by the American Association for Clinical Chemistry.