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Clinical Chemistry 13: 970-975, 1967;
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Clinical Chemistry, Vol 13, 970-975, Copyright © 1967 by the American Association for Clinical Chemistry

Analyses on Heat-coagulated Blood and Serum

The Determination of Creatinine

Morris London 1, Ida A. Freiberger 1, and Jesse H. Marymont Jr. 2

1 Mildred Werner Cancer Research Laboratory, Division of Laboratories, North Shore Hospital, Manhasset, N. Y. 11030.
2 Department of Laboratories, Wesley Medical Center, Wichita, Kan. 67214.

The "standard" heat-clot method of London and Marymont is shown here to be applicable to the determination of creatinine. With this method creatinine can be assayed in very small quantities of serum, and only a conventional laboratory reagent, alkaline picrate, is required. A new fast technic has been developed in which extraction of the clot is carried out for only 10 min. at a high temperature. In addition, the heat-clot method used in conjunction with Lloyd's reagent lends itself to the determination of "true" creatinine values.

Submitted on March 10, 1967
Accepted on May 9, 1967






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Copyright © 1967 by the American Association for Clinical Chemistry.