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Clinical Chemistry 13: 976-984, 1967;
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Clinical Chemistry, Vol 13, 976-984, Copyright © 1967 by the American Association for Clinical Chemistry

Comparison of Methods for Pregnanediol Determination

Edward E. Wallach 1 and Joseph C. Touchstone 2

1 Josiah Macy, Jr., Foundation Fellow.
2 Department of Obstetrics and Gynecology, University of Pennsylvania School of Medicine, Philadelphia, Pa. 19104.

A gas chromatographic method of pregnanediol determination has been carried out on 24-hr. urine specimens collected during various phases of the menstrual cycle and during pregnancy. Pregnanediol values were correlated with endometrial histology and cycle day in nonpregnant subjects, and with duration of gestation during pregnancy. Determinations were performed on free pregnanediol and on the acetylated derivative, both with and without the inclusion of a permanganate wash. One patient was followed through 2 ovulatory cycles with 24-hr. urinary pregnanediol determinations, using gas-liquid chromatography of the free compound. Comparisons were also made between pregnanediol values as determined by gas chromatography and by column chromatography.

The method using gas chromatography is rapid and reproducible, yielding results comparable to those obtained using a colorimetric technic. The gas chromatographic method is applicable during pregnancy, and also in the nonpregnant state for evaluating the occurrence of ovulation. There appears to be no advantage in the performance of a permanganate oxidation or in the production of the diacetate derivative.

Submitted on June 23, 1966
Accepted on May 22, 1967






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Copyright © 1967 by the American Association for Clinical Chemistry.