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Clinical Chemistry 13: 126-131, 1967;
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Clinical Chemistry, Vol 13, 126-131, Copyright © 1967 by the American Association for Clinical Chemistry

Rapid Fluorometric Assay for Plasma Warfarin

Milton Corn 1 and Robert Berberich 2

1 Department of Hematology, George Washington University Medical Division, D. C. General Hospital, Washington, D. C. 20003.
2 Laboratory of Molecular Biology, National Institutes of Health, Bethesda, Md.

Acetone alters the ultraviolet absorption characteristics of warfarin (3-(alpha-acetonylbenzyl)-4-hydroxycoumarin) and increases the intensity of fluorescent emission. Fluorescence of warfarin in acetone is markedly decreased by acidification. These phenomena provide the basis for a rapid, simple assay for plasma warfarin.

Submitted on September 26, 1966
Accepted on October 11, 1966




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Copyright © 1967 by the American Association for Clinical Chemistry.