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Clinical Chemistry 13: 163-166, 1967;
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Clinical Chemistry, Vol 13, 163-166, Copyright © 1967 by the American Association for Clinical Chemistry

A Source of Error in an AutoAnalyzer Determination of Serum Iron

Arthur L. Babson 1 and Norman M. Kleinman 1

1 Warner-Lambert Research Institute, Morris Plains, N. J. 07950.

An AutoAnalyzer method for serum iron was found to be giving erroneously high values owing to a Donnan equilibrium effect on the rate of dialysis of ferrous ions. The addition of neutral salt to the reagent corrected the deficiency. The validity of other assays on the AutoAnalyzer that measure dialyzable ions in the presence of relatively high concentrations of protein should be verified in view of this finding.

Submitted on August 29, 1966
Accepted on September 27, 1966






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Copyright © 1967 by the American Association for Clinical Chemistry.