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Clinical Chemistry 13: 175-185, 1967;
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Clinical Chemistry, Vol 13, 175-185, Copyright © 1967 by the American Association for Clinical Chemistry

Determination of Glutamic Oxalacetic Transaminase Activity by Coupling of Oxalacetate with Diazonium Salts

Sylvan M. Sax 1 and John J. Moore 1

1 Pathology Department, The Western Pennsylvania Hospital, Pittsburgh, Pa. 15224.

A method for measuring serum glutamic oxalacetic transaminase activity is described, in which substrate concentrations are more nearly optimal than in previous colorimetric methods. By coupling the diazonium salt at a pH of 4.2, interference from alpha-oxoglutarate is negligible. Oxalacetate is produced at a linear rate to 180 I.U. Results correlate well with those obtained by a reference spectrophotometric method.

Submitted on August 3, 1966
Accepted on October 27, 1966






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Copyright © 1967 by the American Association for Clinical Chemistry.