Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 13: 215-219, 1967;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heirwegh, K. P. M.
Right arrow Articles by Fevery, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heirwegh, K. P. M.
Right arrow Articles by Fevery, J.

Clinical Chemistry, Vol 13, 215-219, Copyright © 1967 by the American Association for Clinical Chemistry

Determination of Unconjugated and Total N-Acetyl-p-aminophenol (NAPA) in Urine and Serum

Karel P. M. Heirwegh 1 and Johan Fevery 2

1 Laboratory for time Physio-Pathological Study of the Liver, Department for Internal Medicine, St. Rafael's Klinieken, Leuven, Belgium.
2 National Fonds voor Wetenschappelijk Onderzoek van Beigië

A sensitive and accurate method is described for the determination of N-acetyl-p-aminophenol (NAPA) and its metabolites in urine and serum. In strongly acidic medium, p-aminophenol (PAP) resulting from differential extraction and acid hydrolysis of total NAPA and unconjugated NAPA, is diazotized and the diazonium salt coupled with N-(1-naphthyl)ethylenediamine (NED) in the presence of ethanol. The blue azo dye formed is determined spectrophotometrically. Application to liver disease is briefly reported.

Submitted on April 12, 1966
Accepted on August 31, 1966






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1967 by the American Association for Clinical Chemistry.