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Clinical Chemistry 13: 233-241, 1967;
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Clinical Chemistry, Vol 13, 233-241, Copyright © 1967 by the American Association for Clinical Chemistry

Automated Method for the Determination of Serum Creatine Kinase Activity

Gerard A. Fleisher 1

1 Mayo Clinic and Mayo Foundation : Section of Biochemistry, Rochester, Minn. 55901.

An automated (AutoAnalyzer) method for the colorimetric determination of creatine kinase activity in serum is described. This method includes reactivation of creatine kinase with cysteine, incubation of the active enzyme with creatine phosphate and adenosine diphosphate at 37.5°, and subsequent inactivation of enzyme and binding of cysteine by phenylmercuric borate. The enzymatically produced creatine is dialyzed against a solution of diacetyl and reacted with alpha-naphthol in an alkaline solution. The absorbance of the colored end product is measured at 550 mµ. Individual blanks are determined in the absence of adenosine diphosphate. Comparison of results obtained by this method and a manual procedure shows satisfactory agreement.

Submitted on August 9, 1966
Accepted on November 16, 1966






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Copyright © 1967 by the American Association for Clinical Chemistry.