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Clinical Chemistry 13: 261-269, 1967;
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Clinical Chemistry, Vol 13, 261-269, Copyright © 1967 by the American Association for Clinical Chemistry

Alkaline Degradation of Inulin and Its Structural Implications

J. N. BeMiller 1, T. R. Steinheimer 1, and Earl E. Allen Jr. 1

1 Department of Chemistry, Southern Illinois University, Carbondale, Ill.

Alkaline degradation of inulin under controlled conditions shows that not all molecules in an inulin preparation are nonreducing and, thus, alkali-stable and suitable for clinical measurements. Reducing "inulin" molecules are degraded by the normal beta-alkoxy carbonyl mechanism, and the main product of degradation in calcium hydroxide solution is "alpha"-D-glucosaccharinic acid. An inulin preparation can be made alkali- stable by sodium borohydride reduction. There appears to be a difference in the alkali lability of inulin and other fructan preparations obtained from different sources.

Submitted on June 23, 1966
Accepted on August 8, 1966






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Copyright © 1967 by the American Association for Clinical Chemistry.