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Clinical Chemistry 13: 270-275, 1967;
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Clinical Chemistry, Vol 13, 270-275, Copyright © 1967 by the American Association for Clinical Chemistry

Automated Determination of Serum Glutamic Oxaloacetic Transaminase

II. Procedure for the Robot Chemist

Stanley Morgenstern 1, James H. Kaufman 2, and Bernard Klein 2

1 Technicon Instrument Corp., Ardsley, N. V.
2 Automation Research Laboratory, Veterans Administration Hospital, Bronx, N. Y. 10468.

A new and sensitive procedure is described for the automated determination of serum glutamic oxaloacetic transaminase using the Robot Chemist. Enzymically generated oxaloacetic acid is directly coupled with the diazonium salt, fast ponceau L, to form a colored solution whose absorbance is measured at 455 mµ. Correlations are presented between this method and the AutoAnalyzer procedure using the same reagent.

Submitted on June 9, 1966
Accepted on November 16, 1966






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Copyright © 1967 by the American Association for Clinical Chemistry.