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Clinical Chemistry 13: 290-298, 1967;
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Clinical Chemistry, Vol 13, 290-298, Copyright © 1967 by the American Association for Clinical Chemistry

Automated Alkaline Phosphatase Determination

III. Evaluation of Phenolphthalein Monophosphate

Bernard Klein 1 and James H. Kaufman 2

1 Automation Research Laboratory, Veterans Administration Hospital, Bronx, N. Y. 10468.
2 Automation Research Laboratory, Veterans Administration Hospital, Bronx, N. Y. 10468.

An automated procedure for the determination of serum alkaline phosphatase uses phenolphthalein monophosphate as the substrate. Important assets of this substrate are: (1) A chromogenic product is enzymically produced and can be measured directly; (2) Bilirubin does not interfere, thus eliminating the need for a control analysis. Excellent agreement is obtained when the enzyme activities determined by the present method and by the automated p-nitrophenylphosphate procedure are compared.

Submitted on July 22, 1966
Accepted on October 4, 1966






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Copyright © 1967 by the American Association for Clinical Chemistry.