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Clinical Chemistry 13: 773-787, 1967;
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Clinical Chemistry, Vol 13, 773-787, Copyright © 1967 by the American Association for Clinical Chemistry

Application of Thin-layer Chromatography to the Quantitation of Plasma Neutral Lipids and Free Fatty Acids

Robert T. Louis-Ferdinand 1, Donald G. Therriault 1, William F. Blatt 1, Milton Mager 1, and Edward J. Metheson 1

1 Biochemistry-Pharmacology Division, U. S. Army Research Institute of Environmental Medicine, Natick, Mass. 01760.

Neutral lipids and free fatty acids were extracted from plasma and separated on thin-layer chromatoplates coated with silica gel G. The plates were charred by heating after spraying with dichromate-sulfuric acid, and then evaluated densitometrically. Plasma analyses obtained by this procedure were compared with parallel determinations performed by established chemical technics.

Submitted on January 20, 1967
Accepted on March 18, 1967






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Copyright © 1967 by the American Association for Clinical Chemistry.