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Clinical Chemistry 14: 1-5, 1968;
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Clinical Chemistry, Vol 14, 1-5, Copyright © 1968 by the American Association for Clinical Chemistry

A Simplified Method for Plasma Zinc Determination by Atomic Absorption Spectrophotometry

Betty M. Hackley 1, James C. Smith 1, and James A. Halsted 1

1 Nutrition Research Laboratory, Veterans Administration Hospital, Washington, D. C. 20422, and the Departments of Biochemistry and of Epidemiology and Environmental Health, George Washington University School of Medicine, Washington, D. C.

A modified method is described for zinc determination in plasma by atomic absorption spectrophotometry using the Boling three-slot burner. Accurate analysis for the element was accomplished after merely diluting the plasma with deionized water (1 part plasma to 1 part water). Sample preparation time was thus greatly decreased. It was found that standards made up in water resulted in an error of 33%. However, standards prepared in 3% dextran solution gave results similar to those obtained with trichloracetic acid filtrates and water standards. Dextran was found not to be contaminated with zinc. The recovery of zinc added to plasma averaged 98.9%. Because of the simplicity of the method, sources of error and possibilities for contamination were reduced.

Submitted on May 11, 1967
Accepted on July 17, 1967




The following articles in journals at HighWire Press have cited this article:


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 ANN INTERN MEDHome page
C. J. CONDON and R. M. FREEMAN
Zinc Metabolism in Renal Failure
Ann Intern Med, October 1, 1970; 73(4): 531 - 536.
[Abstract] [PDF]

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 Arch Intern MedHome page
K. Mansouri, J. A. Halsted, and Ervin A.
Zinc, Copper, Magnesium, and Calcium in Dialyzed and Nondialyzed: Uremic Patients
Arch Intern Med, January 1, 1970; 125(1): 88 - 93.
[Abstract] [PDF]


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