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Clinical Chemistry 14: 22-30, 1968;
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Clinical Chemistry, Vol 14, 22-30, Copyright © 1968 by the American Association for Clinical Chemistry

Isolation of Albumin from Human Serum by Means of Trichloroacetic Acid and Ethanol

A Comparison of Methods

Takuzo Iwata 1, Hiromi Iwata 1, and James F. Holland 1

1 Department of Medicine A, Roswell Park Memorial Institute, Buffalo, N. Y. 14203.

A rapid method for nearly quantitative isolation of human serum albumin is described. The present technic (1) is a modification of the Schwert method (2). In the present method 4 ml. of serum is precipitated with trichloroacetic acid in a final concentration of 5% (w/v). The precipitate is washed with TCA, and after aqueous suspension, ethanol is added to a final concentration of 80%. The undissolved globulin is washed with ethanol and the solubilized albumin used for quantification, characterization, and isotope counting.

Recovery of added human serum albumin was approximately 98%; recovery of added 131I albumin was 90%.

Cellulose acetate electrophoresis showed a single band, and agar diffusion produced one precipitate line. Immunoelectrophoresis with anti-human serum revealed one or two very faint arcs in the agr-, or agr- and beta-globulin regions, in addition to the major arc of albumin.

The method has shown excellent reproducibility in replicate analyses of normal and pathologic serums.

Submitted on December 2, 1965
Accepted on July 17, 1967




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Copyright © 1968 by the American Association for Clinical Chemistry.