Clinical Chemistry
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Clinical Chemistry 14: 35-37, 1968;
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Clinical Chemistry, Vol 14, 35-37, Copyright © 1968 by the American Association for Clinical Chemistry

Simultaneous Determination of Pregnanediol and Pregnanolone in Urinary Extracts by Gas Chromatography

M. Guarnieri 1 and R. D. Barry 1

1 Institute of Nutrition and Food Technology, and the Departments of Physiological Chemistry, and Obstetrics and Gynecology, Ohio State University, Columbus, Ohio 43210.

A method for the simultaneous determination of pregnanediol and pregnanolone in urinary extracts by gas chromatography is described. The method involves prior enzymatic hydrolysis of the steroid conjugates, chloroform extraction, and quantitation by means of gas chromatography.

Submitted on March 6, 1967
Accepted on April 12, 1967







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Copyright © 1968 by the American Association for Clinical Chemistry.