Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 14: 47-57, 1968;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Romel, W. C.
Right arrow Articles by DuFrene, J. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Romel, W. C.
Right arrow Articles by DuFrene, J. K.

Clinical Chemistry, Vol 14, 47-57, Copyright © 1968 by the American Association for Clinical Chemistry

Detection of Serum Alkaline Phosphatase Isoenzymes with Phenolphthalein Monophosphate Following Cellulose Acetate Electrophoresis

William C. Romel 1, S. J. LaMancusa 1, and John K. DuFrene 1

1 Department of Chemistry, North Las Vegas Hospital, Inc., 1401 E. Lake Mead Blvd., North, Las Vegas, Nev. 89031.

Serum containing normal and abnormal levels of alkaline phosphatase activity were assayed for total enzyme activity, then fractionated by electrophoresis on cellulose acetate membranes for 20 min. The new substrate, phenolphthalein monophosphate, was employed to locate the isoenzymes on the cellulose acetate membranes and to measure their activity by eluting and scanning procedures. The sensitivity and precision of both technics are presented.

Submitted on January 27, 1966
Accepted on August 4, 1967






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1968 by the American Association for Clinical Chemistry.