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Clinical Chemistry 14: 47-57, 1968;
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Clinical Chemistry, Vol 14, 47-57, Copyright © 1968 by the American Association for Clinical Chemistry

Detection of Serum Alkaline Phosphatase Isoenzymes with Phenolphthalein Monophosphate Following Cellulose Acetate Electrophoresis

William C. Romel 1, S. J. LaMancusa 1, and John K. DuFrene 1

1 Department of Chemistry, North Las Vegas Hospital, Inc., 1401 E. Lake Mead Blvd., North, Las Vegas, Nev. 89031.

Serum containing normal and abnormal levels of alkaline phosphatase activity were assayed for total enzyme activity, then fractionated by electrophoresis on cellulose acetate membranes for 20 min. The new substrate, phenolphthalein monophosphate, was employed to locate the isoenzymes on the cellulose acetate membranes and to measure their activity by eluting and scanning procedures. The sensitivity and precision of both technics are presented.

Submitted on January 27, 1966
Accepted on August 4, 1967






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Copyright © 1968 by the American Association for Clinical Chemistry.