Clinical Chemistry
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Clinical Chemistry 14: 6-11, 1968;
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Clinical Chemistry, Vol 14, 6-11, Copyright © 1968 by the American Association for Clinical Chemistry

Fluorometric Determination of Zinc in Biologic Fluids

D. Mahanand 1 and J. C. Houck 1

1 Department of Neurology and the Clinical Research Center, Children’s Hospital, Washington, D. C. 20009.

The 8-quinolinol complexes of zinc at pH 8.0 (in universal buffer) are stabilized by gum arabic and demonstrate a marked fluorescence at 517 mµ when excited at 375 mµ. Under these conditions only zinc—not magnesium or calcium at physiologic concentrations—can be determined in plasma and urine. Adult plasma has 94 ± 7 µg. zinc per 100 ml. while the plasma of children contains 108 ± 15g./100 ml.

Submitted on July 5, 1967
Accepted on August 14, 1967






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Copyright © 1968 by the American Association for Clinical Chemistry.