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Clinical Chemistry 14: 1023-1025, 1968;
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Clinical Chemistry, Vol 14, 1023-1025, Copyright © 1968 by the American Association for Clinical Chemistry

A Simple Method for the Determination of Lipoprotein Lipase

Leonard J. Stutman 1 and Marilyn Dolliver 1

1 Coagulation Research Laboratory, St. Vincent’s Hospital and Medical Center, New York, N. Y. 10011.

A rapid method for determining the in-vivo effect of small amounts of intravenous heparin utilizing thin-layer chromatography is presented. A dense band of stainable fatty acids appears and represents nonesterified acids after the fatty acid esters have been hydrolyzed by lipoprotein lipase and determines, therefore, whether the enzyme is activated by heparin.

Submitted on March 4, 1968
Accepted on April 11, 1968






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Copyright © 1968 by the American Association for Clinical Chemistry.