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Clinical Chemistry 14: 960-966, 1968;
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Clinical Chemistry, Vol 14, 960-966, Copyright © 1968 by the American Association for Clinical Chemistry

Method for Fully Automated Determination of Total Cholesterol in Blood Serum, Including Saponification and Extraction

J. van der Honing 1, C. C. Saarloos 1, and J. Stip 1

1 Unilever Research Laboratory, Olivier van Noortlaan 120, Vlaardingen, The Netherlands.

A fully automated method has been developed for the determination of total cholesterol in blood serum, using the AutoAnalyzer system. According to the new method, based on the cholesterol determination of Abell et al. (1), the serum sample is saponified and subsequently extracted with carbon tetrachloride. After treatment with Liebermann-Burchard reagent, the amount of cholesterol is determined at 630 nm.

The method can be used for free and esterified cholesterol because saponification is carried out. The relationship between the new method and that of Abell et al. is linear. The correlation coefficient is 0.98 and the standard error 1.5%. According to the new method, 30 samples can be analyzed per hour.

Submitted on August 30, 1967
Accepted on February 16, 1968






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Copyright © 1968 by the American Association for Clinical Chemistry.