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Clinical Chemistry 14: 1074-1079, 1968;
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Clinical Chemistry, Vol 14, 1074-1079, Copyright © 1968 by the American Association for Clinical Chemistry

Freezing as a Method of Preserving Blood Ammonia

William C. Lowe 1

1 Medical Service, Veterans Administration Hospital, East Orange, N. J. 07019; and the Department of Medicine, New Jersey College of Medicine.

The influence of temperature on in-vitro generation of ammonia in whole blood was evaluated. It was found that when blood was placed in a freezer at -20 to -25° there was no significant rise of blood ammonia levels even after 8 days of storage. There was a gradual rise when blood was placed in a refrigerator with the temperature at about 4° and a more rapid rise at room temperature. The inhibition of ammonia production at a temperature below -20° facilitates the laboratory procedure when it is necessarily delayed.

Submitted on February 21, 1968
Accepted on March 12, 1968






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Copyright © 1968 by the American Association for Clinical Chemistry.