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Clinical Chemistry 14: 1091-1096, 1968;
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Clinical Chemistry, Vol 14, 1091-1096, Copyright © 1968 by the American Association for Clinical Chemistry

Rapid Methods for the Determination of Pregnanediol in Urine

P. J. Brombacher 1, A. H. J. Gijzen 1, and P. E. Verheesen 1

1 Department of Clinical Chemistry, St. Joseph Hospital, Heerlen, The Netherlands.

Pregnanediol determination in urine was performed by alumina-column chromatography followed by reaction with sulfuric acid and by gas chromatography. After enzymatic hydrolysis of the conjugated urinary steroids, there is a very good agreement between both methods.

Submitted on January 18, 1968
Accepted on March 10, 1968






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Copyright © 1968 by the American Association for Clinical Chemistry.