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Clinical Chemistry 14: 1097-1106, 1968;
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Clinical Chemistry, Vol 14, 1097-1106, Copyright © 1968 by the American Association for Clinical Chemistry

A New Rapid Method for the Determination of Serum Lipase

Philip H. Dirstine 1, Charles Sobel 1, and Richard J. Henry 1

1 Bio-Science Laboratories, 7600 Tyrone Ave., Van Nuys, Calif. 91405.

A new method is described for the determination of serum lipase using olive oil as substrate, and photometric, copper soap analysis for the measurement of fatty acids formed. The great sensitivity of this technic for measurement of the fatty acids permits a 30-min. incubation time and the use of 0.1 ml. serum. The reaction is zero order for this time period. Other aspects of the enzyme kinetics were studied and the normal values determined.

Submitted on March 9, 1968
Accepted on April 1, 1968






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Copyright © 1968 by the American Association for Clinical Chemistry.