Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 14: 1160-1171, 1968;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Savory, J.
Right arrow Articles by Sunderman, F. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Savory, J.
Right arrow Articles by Sunderman, F. W., Jr.

Clinical Chemistry, Vol 14, 1160-1171, Copyright © 1968 by the American Association for Clinical Chemistry

A Biuret Method for Determination of Protein in Normal Urine

John Savory 1, Pin H. Pu 1, and F. William Sunderman Jr. 1

1 Pathology Department, University of Florida College of Medicine, Gainesville, Fla. 32601.

A biuret method has been developed which provides quantitative measurements of protein in normal urine without interference from drugs or pigments. This method is intended for use in monitoring clinical trials of new drugs—to detect nephrotoxicity. Protein is precipitated from duplicate samples of urine by addition of cold ethanolic phosphotungstic acid. The protein precipitates are separated by centrifugation and washed with ethanol. Protein from one of the duplicate samples is dissolvel in biuret reagent. Protein from the second sample is dissolved in an alkaline tartrate reagent which is identical to the biuret reagent, excepting that copper sulfate has been omitted. After 20 min., the differential absorbance of the two samples is measured at 540 mµ. The limit of sensitivity for detection of protein in urine is 0.5 mg./100 ml. The coefficient of variation of replicate analyses of protein in normal urine is 4.2%. The recovery of protein added to urine averages 103 ± 3%. Analyses of urinary protein by the biuret procedure provide close correlation with measurements by an amido black staining method. Systematic search has failed to reveal interference from urinary pigments, compounds, or drugs which are normally or occasionally encountered in hospitalized patients. In 24-hr. collections of urine from 28 healthy adults, the protein concentrations averaged 6.2 mg./100 ml. (range 3.0-12.2), and the protein excretions averaged 77 mg./day (range 40-150).

Submitted on February 2, 1968
Accepted on March 31, 1968




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
G. L. Hortin and B. Meilinger
Cross-Reactivity of Amino Acids and Other Compounds in the Biuret Reaction: Interference with Urinary Peptide Measurements
Clin. Chem., August 1, 2005; 51(8): 1411 - 1419.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
F. M. Yilmaz, N. Celebi, and D. Yucel
Automated Turbidimetric Benzalkonium Chloride Method for Measurement of Protein in Urine and Cerebrospinal Fluid
Clin. Chem., August 1, 2004; 50(8): 1450 - 1452.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
T. Marshall and K. M. Williams
Total Protein Determination in Urine: Elimination of a Differential Response between the Coomassie Blue and Pyrogallol Red Protein Dye-binding Assays
Clin. Chem., March 1, 2000; 46(3): 392 - 398.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
P. L. M. Lynch, J. Savory, and D. M. Haverstick
Urine Total Protein Measurement with the Vitros Dry Reagent Technology: Modification of Diluent to Resolve Positive Bias of Diluted Samples
Clin. Chem., March 1, 1998; 44(3): 674 - 675.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1968 by the American Association for Clinical Chemistry.