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Clinical Chemistry 14: 1206-1210, 1968;
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Clinical Chemistry, Vol 14, 1206-1210, Copyright © 1968 by the American Association for Clinical Chemistry

Comparison of Atomic Absorption Spectrophotometry and Fluorometry for the Measurement of Serum Calcium

Denis 0. Rodgerson 1 and Iris K. Moran 1

1 Department of Pediatrics, University of Colorado Medical Center, Denver, Colo. 80220.

An atomic absorption spectrophotometric (AAS) and a fluorometric method for the measurement of serum calcium were found to be comparable in acceptable reproducibility. The recovery figures obtained by the fluorometric procedure were considerably lower than those given by AAS in the 2-4 mEq./L. range, although recoveries at higher levels (6 mEq./L.) were acceptable. The average recovery for the fluorometric method throughout the range 2-6 mEq./L. was 83.8%; for AAS, 98.9%. Marked interference was observed in the fluorometric method when hemolyzed or icteric serum was assayed. In four groups of hospital patients—premature infants, newborn infants, older children, and adults—AAS gave ranges for serum calcium values which were higher than those obtained by fluorometry.

Submitted on March 21, 1968
Accepted on May 18, 1968






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Copyright © 1968 by the American Association for Clinical Chemistry.