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Clinical Chemistry 14: 116-131, 1968;
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Clinical Chemistry, Vol 14, 116-131, Copyright © 1968 by the American Association for Clinical Chemistry

A New and Rapid Method for the Determination of Glucose by Measurement of Rate of Oxygen Consumption

Arnold Henry Kadish 1, Robert L. Litle 1, and James C. Sternberg 1

1 Division of Medicine, Mount Sinai Division, Cedars-Sinai Medical Center, Los Angeles, Calif. 90048; and from the Research Departmnent, Scientific Instruments Operation, Beckman Instruments, Inc., Fullerton, Calif. 92634.

Glucose levels in serum, plasma, and urine are determined rapidly and conveniently by a new glucose oxidase method employing a polarographic oxygen sensor with a circuit modified to record the rate of oxygen consumption. The maximum apparent rate of oxygen consumption relative to the rate obtained with a glucose standard provides a direct measure of the glucose level in the sample; results are obtainable within 20 sec. after sample (100 µl.) addition and within 3 min. after a blood sample is withdrawn from a patient. Interferences associated with prior colorimetric glucose oxidase methods are avoided by measuring oxygen consumption instead of hydrogen peroxide formation. The method is described and results are presented showing a standard deviation of less than 1.5% on replicate determinations and a bias of 1% with respect to data obtained on the same samples by the automated ferricyanide method.

Submitted on May 8, 1967




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[Abstract] [Full Text]

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 Arch Pediatr Adolesc MedHome page
R. Zannolli, A. Rebeggiani, F. Chiarelli, and G. Morgese
Hyperinsulinism as a Marker in Obese Children
Arch Pediatr Adolesc Med, August 1, 1993; 147(8): 837 - 841.
[Abstract] [PDF]

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 ANN INTERN MEDHome page
J. H. Warram, B. C. Martin, A. S. Krolewski, J. S. Soeldner, and C. R. Kahn
Slow Glucose Removal Rate and Hyperinsulinemia Precede the Development of Type II Diabetes in the Offspring of Diabetic Parents
Ann Intern Med, December 15, 1990; 113(12): 909 - 915.
[Abstract] [PDF]

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 Arch Intern MedHome page
M. M-T. Fuh, S-M. Shieh, D-A. Wu, Y-D. I. Chen, and G. M. Reaven
Abnormalities of Carbohydrate and Lipid Metabolism in Patients With Hypertension
Arch Intern Med, June 1, 1987; 147(6): 1035 - 1038.
[Abstract] [PDF]

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 Arch Intern MedHome page
C. K. Lardinois, G. C. Liu, and G. M. Reaven
Glyburide in Non-Insulin-Dependent Diabetes: Its Therapeutic Effect in Patients With Disease Poorly Controlled by Insulin Alone
Arch Intern Med, June 1, 1985; 145(6): 1028 - 1032.
[Abstract] [PDF]

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 Arch Intern MedHome page
G. C. Liu, A. M. Coulston, C. K. Lardinois, C. B. Hollenbeck, J. G. Moore, and G. M. Reaven
Moderate Weight Loss and Sulfonylurea Treatment of Non--insulin-dependent Diabetes Mellitus: Combined Effects
Arch Intern Med, April 1, 1985; 145(4): 665 - 669.
[Abstract] [PDF]

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 Arch OphthalmolHome page
B. C. Lerner, S. D. Varma, and R. D. Richards
Polyol Pathway Metabolites in Human Cataracts: Correlation of Circulating Glycosylated Hemoglobin Content and Fasting Blood Glucose Levels
Arch Ophthalmol, June 1, 1984; 102(6): 917 - 920.
[Abstract] [PDF]

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 Arch Intern MedHome page
C. K. Lardinois, M. S. Greenfield, H. C. Schwartz, H. J. Vreman, and G. M. Reaven
Acarbose Treatment of Non-Insulin-Dependent Diabetes Mellitus
Arch Intern Med, February 1, 1984; 144(2): 345 - 347.
[Abstract] [PDF]

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 Arch Pediatr Adolesc MedHome page
J. I. Wolfsdorf, A. Sadeghi-Nejad, and B. Senior
Ketonuria Does Not Exclude Hyperinsulinemic Hypoglycemia
Arch Pediatr Adolesc Med, February 1, 1984; 138(2): 168 - 171.
[Abstract] [PDF]

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 Arch Intern MedHome page
M. S. Greenfield, L. Doberne, M. Rosenthal, H. J. Vreman, and G. M. Reaven
Lipid Metabolism in Non-Insulin-Dependent Diabetes Mellitus: Effect of Glipizide Therapy
Arch Intern Med, August 1, 1982; 142(8): 1498 - 1500.
[Abstract] [PDF]

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 Arch OphthalmolHome page
C. W. Jones, J. G. Cunha-Vaz, and M. M. Rusin
Vitreous Fluorophotometry in the Alloxan- and Streptozocin-Treated Rat
Arch Ophthalmol, July 1, 1982; 100(7): 1141 - 1145.
[Abstract] [PDF]

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 Arch SurgHome page
L. H. Toledo-Pereyra, K. D. Valgee, J. Castellanos, and M. Chee
Hypothermic Pulsatile Perfusion: Its Use in the Preservation of Pancreases for 24 to 48 Hours Before Islet Cell Transplantation
Arch Surg, January 1, 1980; 115(1): 95 - 98.
[Abstract] [PDF]

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 Arch SurgHome page
L. H. Toledo-Pereyra, J. Castellanos, G. Manifacio, and R. C. Lillehei
Basic Requirements of Pancreatic Mass for Transplantation
Arch Surg, September 1, 1979; 114(9): 1058 - 1062.
[Abstract] [PDF]

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 ScienceHome page
D. Thompson and R. Campbell
Hunger in humans induced by 2-deoxy-D-glucose: glucoprivic control of taste preference and food intake
Science, December 9, 1977; 198(4321): 1065 - 1068.
[Abstract] [PDF]

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 Arch SurgHome page
D. O. Ferris, G. D. Molnar, N. Schnelle, J. D. Jones, and E. A. Moffitt
Recent Advances in Management of Functioning Islet Cell Tumor
Arch Surg, April 1, 1972; 104(4): 443 - 446.
[Abstract] [PDF]


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