Clinical Chemistry
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Clinical Chemistry 14: 145-155, 1968;
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Clinical Chemistry, Vol 14, 145-155, Copyright © 1968 by the American Association for Clinical Chemistry

Radioimmunoassay of Human Growth Hormone

Mechthilde Knoller 1, M. U. Tsao 1, and George H. Lowrey 1

1 Department of Pediatrics and Communicable Diseases, University of Michigan Medical Center, Ann Arbor, Mich. 48104.

Detailed methods for: (1) 131I iodination of human growth hormone (HGH); (2) purification of labeled HGH (HGH-131I; and (3) radioimmunoassay of HGH are given. Bio-Gel filtration is introduced as a rapid and reproducible method for purifying HGH-131I which has been obtained by a modification of the method of Greenwood et al. (1). Highly purified HGH-131I with a bindability of 96% or more is usually achieved.

Submitted on May 24, 1967
Accepted on August 4, 1967




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Copyright © 1968 by the American Association for Clinical Chemistry.