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Clinical Chemistry 14: 179-184, 1968;
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Clinical Chemistry, Vol 14, 179-184, Copyright © 1968 by the American Association for Clinical Chemistry

Phenothiazine Interference and the Porter-Silber Reaction

Aaron Lupovitch 1

1 Department of Pathology, William Beaumont Hospital, Royal Oak, Mich. 48072

Phenothiazines interfere with the Porter-Silber reaction for urinary 17, 21-dihydroxy, 20-ketosteroids. The present study suggests that the interference is a depression or inhibition of the formation of the corticosteroid bisphenylhydrazone. Slight-to-marked aberration in the color of the acidified urine extract from dark yellow to orange or pink is the clue indicating that interference is present.

Submitted on July 5, 1967
Accepted on August 28, 1967






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Copyright © 1968 by the American Association for Clinical Chemistry.