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Clinical Chemistry 14: 208-211, 1968;
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Clinical Chemistry, Vol 14, 208-211, Copyright © 1968 by the American Association for Clinical Chemistry

Preparation of Bilirubin-Diglucuronide Concentrates from Dog Bladder Bile

Edmund Talafant 1 and Jirí Appelt 1

1 Department of Medical Chemistry, University J. Ev. Purkynecaron, Komenského nám. 2, Brno, Czechoslovakia.

A method is described for the preparation of bile concentrate that contains a high proportion of bilirubin diglucuronide. The method involves acetone fractionation, precipitation as cinchonidine adsorbate, reprecipitation as lead salt, and finally conversion to a sodium salt by means of cationic exchange resin. The properties of the final concentrate are given.

Submitted on July 5, 1967






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Copyright © 1968 by the American Association for Clinical Chemistry.