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Clinical Chemistry 14: 222-238, 1968;
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Clinical Chemistry, Vol 14, 222-238, Copyright © 1968 by the American Association for Clinical Chemistry

Estimation of Creatinine by the Jaffe Reaction

A Comparison of Three Methods

Harry Husdan 1 and Abraham Rapoport 1

1 Metabolic-Renal Unit and the Department of Biochemistry, Toronto Western Hospital, and the Departments of Medicine and of Pathological Chemistry, University of Toronto, Toronto, Canada.

Total chromogen, true, and AutoAnalyzer methods of measuring serum and urine creatinine by the Jaffe reaction were investigated. Some factors influencing this reaction were examined. These included wavelength, blank, linearity, and conditions of color development. Modifications of the three methods were made and their precision, recovery, and sample stability determined. The interference of ketones and glucose were measured. Finally, the values obtained by the three methods on the same samples of serum and urine were compared statistically.

Submitted on July 5, 1967




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